A study on the characterization of infection of PEGylated adenovirus

• Main Authors: Jun-Ren Huang, 黃俊仁
• Other Authors: Shih-Yow Huang
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/04247336232315473238

碩士 === 國立臺灣大學 === 化學工程學研究所 === 91 === PEGylation is the covalent attachment of activated monomethoxy polyethylene glycols PEG to free lysine groups of proteins. PEGylated recombinant adenovirus has been shown to reduce humoral immune response and prolong virus viability. In this research, I studied the effects of different degree of PEGylated adenovirus on its infection rate of target cells. I also compared the changes in physical properties and survival of PEGylated adenovirus under environmental stress. Adenovirus with different degree of PEGylation was used in the experiments. As the degree of PEGylation increased, the negative charge on the viral surface decreased and the more virus aggregation. Analyses of infection and attachment kinetics showed that the infection and attachment rates of virus on target cells decreased upon the increase in degree of PEGylation. It indicated that PEG on the surface of adenovirus possessed the shielding effect which resulted in decreasing the association of virus and the receptors on the cell membrane. Furthermore, studies in attachment kinetics suggested that the shielding effect of PEG on fibers of adenovirus may should be the most important factor in determining the infection rate.