| Summary: | 碩士 === 國立海洋大學 === 食品科學系 === 91 === Abstract
For discovering the function of physiological active peptides in the mollusk as reference for further study and development, Haliotis aquatilis, Babylonia areolata (Gastropoda), Meretrix lusoria, Ruditapes variegates (Bivalvia), Loligo edulis Hoyle and Octopus variablilis (Cephalopoda) were focused in this study. First, using boiled water extracted low molecular peptides from mollusk muscle. Then purification process separated these extractives by using Amicon, Sephadex G-25 and CM-sephrose CL-6B. All the steps were monitored antioxidativity: inhibiting effect of lionleic acid autoxidation, reducing power, scavenging effect of DPPH radical and chelating Fe2+ effect, respectively. As result, the inhibiting effect of lionleic acid autoxidation of the extractives (MW<5kD) was shown more obvious than the extractives (MW5~10kD). After chromatographing on Sephadex G-25, the mollusk muscle extractives were found that Cephalopoda extractives and Bivalvia extractives had antioxidativity in fraction No.56-61, No.49-62, respectively; the Haliotis aquatilis extractives had the inhibiting effect of lionleic acid autoxidation and the reducing power in fraction No.58-61, and the scavenging effect of DPPH radical in fraction No.47-57; the Babylonia areolata extractives had the inhibiting effect of lionleic acid autoxidation, the reducing power and the scavenging effect of DPPH radical in fraction No.52-65.
The best antioxidativity in fraction No.56-61, No.49-62, Cephalopoda on Sephadex G-25, was taken further to purify by CM-sephrose CL-6B. Then it was found that the adsorbed peptides have antioxidation. According to amino acid analysis, the peptides might be contained more amino acid which had positive electronic branch chain (such as: Arg, Lys, His).
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