Detection and Analysis of Trimethylamine N-oxide
碩士 === 國立臺灣師範大學 === 化學研究所 === 91 === Trimethylamine and trimethylamine N-oxide (denoted TMAO) are important biological metabolites, widely distributed in marine organisms. TMAO is believed as an oxidation intermediate of trimethylamine. Because of this, the contents of trimethylamine and/...
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| Format: | Others |
| Language: | zh-TW |
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2003
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| Online Access: | http://ndltd.ncl.edu.tw/handle/56583144033339557273 |
| Summary: | 碩士 === 國立臺灣師範大學 === 化學研究所 === 91 === Trimethylamine and trimethylamine N-oxide (denoted TMAO) are important biological metabolites, widely distributed in marine organisms. TMAO is believed as an oxidation intermediate of trimethylamine. Because of this, the contents of trimethylamine and/or TMAO in sea foods are regarded as a symbol of freshness. The detection of TMAO thus becomes an important analytical issue currently.
In this thesis, we successfully prepared phenothiazine-modified electrodes and used them to analyze the concentration of TMAO. Experiment results show that poly(methylene blue) is an effective mediator for the reduction of TMAO. The linear calibration range covers 0.001∼1 M. The detection limit may reach a level of 10-4 M. Poly(methyl violet) also shows a similar catalytic potential. The linear calibration range covers 10μM∼10 mM. The dection limit is about 10μM.
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