Preparation and application of optical isomer piezoelectric crystal sensor for esters based on immobilized C60-lipase enzyme

• Main Author: 陳秋樺
• Other Authors: JENG-SHONG SHIH
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/43028304598181661655

碩士 === 國立臺灣師範大學 === 化學研究所 === 91 === Immobilized fullerene C60－lipase was synthesized and applied in a piezoelectric crystal sensor for optical isomer of esters. The C60 coated piezoelectric quartz crystal sensor was employed to study the interaction between C60 and lipase. The partially irreversible response for lipase was obserred by the desorption study, which implied that lipase could be adsorbed on C60 by chemisorption. Effects of pH and solvents on the response of the C60 coated piezoelectric crystal sensor for lipase was also studied. Optimum pH was found to be at 7 and optimum temperature was 31oC. The reactivity between C60 and lipase was decreased in organic solvents such as methanol and ethanol. The water insoluble C60－lipase was identified with IR spectrography. The C60－lipase coated SiO2 chip with pore size of about 5~10 μm was prepared. The activity of immobilized C60－lipase was investigated and was still activated. The increased concentration of lipase resulted the increase in the ability of C60－lipase catalyze the hydrolysis of L－amino acid esters. The immobilized C60－lipase could catalyze the hydrolysis of L－amino acid esters, but not D－amino acid esters. The C60－cryptand[2,2] coated piezoelectric (PZ) crystal sensor with immobilized C60－lipase was prepared to detect L－amino acid esters in aqueous solutions. The home－made optical isomer PZ sensor for esters was based on the adsorption of L－amino acid, the product of the hydrolysis of L－amino acid ester with C60－lipase, onto the C60－cryptand[2,2] coated quartz crystal. The optical isomer PZ sensor for esters based on C60－lipase was successfully to be applied to distinguish between D－form and L－form amino acid esters. The optical isomer PZ sensor for esters exhibited good sensitivity and reproducibility for L－amino acid esters in aqueous solutions.