The developemnt of benzophenone-based photoimmobilization for the fabrication of protein microarray

• Main Authors: Ying-lan Tsai, 蔡英蘭
• Other Authors: Ruey-an Doong
• Format: Others
• Language: en_US
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/52112878006427527239

碩士 === 國立清華大學 === 原子科學系 === 91 === The development of protein microarray has recently received much attention in diagnosis and pharmaceutics. A key point for the development of protein microarray is the protein immobilization technique. One of the limitations for the developed protein immobilization techniques is nonspecific adsorption. The purpose of this study was to develop the BP-based photoimmobilization combined self-assembled monolayers (SAMs) techniques for protein fixation. The SAM procedures were optimized to minimize the nonspecific adsorption and applied to the fabrication of protein microarray, including protein photopatterning via photomask and protein array with pin-printing method. Surface of SAM was characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), contact angle measurement, and radiolabel assay. Results show that the protein cover density was 1.46 × 1010 molecules/mm2 on the gold surface using 1 mg/ml BP-based photoimmobilibzation technique and the nonspecific adsorption could be minimized to 2 %. The patterning size of 12 mm was obtained with the illumination of UV light using photomask. Combination with pin-printing method, Mouse IgG with 33% glycerol printed to react with antibody was observed as the spot size of 1 mm. The results show that this BP based on photoimmobilization technique has the potential for the fabrication of protein array.