Cloning and Characterization of Lipid Transfer Protein I Genes in Rice (Oryza sativa) and Mungbean (Vigna radiata)

• Main Authors: Kun-Hsiang Liu, 劉坤湘
• Other Authors: 林彩雲
• Format: Others
• Language: en_US
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/87263330793010233372

博士 === 國立清華大學 === 生命科學系 === 91 === ABSTRACT To explore the changes in response to environmental stresses, we isolated a lipid transfer protein (LTP) gene in rice (Oryza sativa) suspension cells in the presence or absence of sucrose by using mRNA differential display. LTPs are small and basic proteins, which consist of eight highly conserved cysteines forming four disulfide bonds. Two main families of LTPs, LTPI and LTPII, are identified with molecular mass of 9 kDa and 7 kDa. LTPs exist in various plants, and the expression of plant LTPs shows temporal and spatial patterns. We found this rice ltp (Osltp) mRNA expressed in developing and fresh seeds, as well as roots in mature plants; and the level of Osltp mRNA increased under water stress, such as high salt, dehydration, low temperature, and abscisic acid (ABA) treatments. Furthermore, we isolated two novel mungbean (Vigna radiata) ltp genes, Vrltp1 and Vrltp2, by screening cDNA library. Both Vrltp1 and Vrltp2 mRNAs expressed in floral buds and only Vrltp1 mRNA expressed in immature seeds. In the vegetative tissues, the Vrltp1 and Vrltp2 mRNAs display their shoot specificity. The levels of Vrltp1 and Vrltp2 mRNAs also increase in response to salt, dehydration and ABA treatments. We assume that these ltp genes participate in the protection from damage under water stress, and the regulatory mechanisms are needed to be elucidated.