| Summary: | 碩士 === 國立清華大學 === 生命科學系 === 91 === Betulinic acid (BA), a triterpenoid of white birch (Betula alba), exhibits anti-viral, anti-malarial, anti-tumor and anti-inflammatory activities. It has been shown to be an inhibitor of bovine pancreatic PLA2 (bp-PLA2); however, its cellular targets remain to be explored. As an effort of identifying the cellular target of BA, the BA immobilized matrix was made and the utility of the immobilized column was studied. Enzymatic activity assay and cytotoxicity test were used to confirm that the physical interaction between BA and PLA2 could change the PLA2 biological function. This interaction may be mediated by electrostatic interaction and hydrophobic interaction dependent on Ca2+. Eventually, the results identified a physical interaction of BA and bp-PLA2 or N-PLA2. It was suggested that this interaction could be applied to PLA2 purification and N-PLA2 toxin neutralization. In this thesis, the BA-immobilized column exhibited an affinity with bp-PLA2 or N-PLA2. Surprisingly, the column also binds cardiotoxin γ, which does not resemble to PLA2 in tertiary structure. The results represent the possibility of the cellular targets of BA besides PLA2. It was proved that BA-immobilized affinity column designed in this thesis might be applied to explore cellular targets of BA.
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