| Summary: | 碩士 === 國立中山大學 === 生物科學系研究所 === 91 === Antrodia camphorata is a Taiwan-unique fungus which grows on the walls inside the core of decayed Cinnamomum kanehirae. Antrodia camphorata provides many bioactive substance and it is cultivated mainly via mycelium nowadays.
First section of this research is focused on Antrodia camphorata (CCRC-35716) in liquid cultivation medium and the changes within extracellular polysaccharides, trierpenoids, pH and growth. It also uses HPLC to analyze the differences of mycelium triterpenoids. This experiment showed when Antrodia camphorata was cultivated in PDB liquid medium for 14 days; it had the highest extracellular polysaccharides (0.23 �b 0.002 g/L) in day 11, the highest dry mycelium (6.19 �b 0.190 g/L)in day 8 and the highest triterpenoids in day 12,the lowest pH (3.20 �b 0.032) in day 8, and the lowest carbon source in day 8 and day 9. pH of 3-6 in most favorable for the growth of mycelium and pH 2 inhibits its growth. When growing at pH 7, triterpenoids volume is at the highest. When cultivating with PDA soil medium, light has negative effects on triterpenoids metabolism. Based on the triterpenoids HPLC analysis chart, Antrodia camphorata, Ganoderma lucidum, Coriolus versicolor, Hericium erinaceum, Armillaria mellea, Cordyceps sinensis, etc. can be identified easily. Difference in Triterpenoids can also be used to analyze other fungi used in medicine.
Second section of this research is to analyze the sequence of mitochondrial rDNA and discuss its phylogeny. By utilizing two mitochondrial DNA sequences, ssu rDNA and lsu rDNA, and Neighbor Jointing and UPGMA method, Phylogeny tree was developed which can be used to identify in molecular biology the Antrodia camphorata in the phylogeny. However, it can not offer effective analysis in identifying relationships among the same species .
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