Comparison of Immunprotectivity Provided by the Conventional and the Baculovirus Expressied VP2 Subunit Protein of Infectious Bursal Disease Virus （IBDV） as Inactivated IBDV Vaccine Antigen in Chickens
碩士 === 國立屏東科技大學 === 生物科技研究所 === 91 === Abstract Infectious bursal disease virus (IBDV) is a immunosuppressive infectious disease in causing necrotic of B-lymphoblast in chickens at 3 to 6 weeks of age. Viral protein (VP) 2 and 3 are the mainly important 2 structural proteins of IBDV. The...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2003
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| Online Access: | http://ndltd.ncl.edu.tw/handle/41798048815901870805 |
| Summary: | 碩士 === 國立屏東科技大學 === 生物科技研究所 === 91 === Abstract
Infectious bursal disease virus (IBDV) is a immunosuppressive infectious disease in causing necrotic of B-lymphoblast in chickens at 3 to 6 weeks of age. Viral protein (VP) 2 and 3 are the mainly important 2 structural proteins of IBDV. The VP2 of IBDV contains the antigenic epitopes in inducing the neutralizing antibody. The whole length of VP2 gene, approximately 1.5 Kb nucleotides, of a recently local isolate (V263/TW02) IBDV being verified with good cross protectivity against several local isolates was amplified with reverse transcriptase-polymerase chain reaction , sequenced and phylogenic analysed of the nucleotide as a very virulent IBDV (vvIBDV) with some point mutation. The gene was then cloned in the vector of the baculovirus expression system for producing the most protective VP2 subunit protein as antigen for IBDV V263/TW02-VP2 subunit vaccine preparation. A3（48 kDa）and C2（21 kDa）2 subunit proteins was found expressied in the system. The V263/TW02-VP2-C2 subunit vaccine was then used to immunized the specific-pathogen-free chickens for comparison of the protectivity with the conventional inactivated V263/TW02 IBDV vaccine against the homologous IBDV challenge. The V263/TW02-VP2 subunit vaccine was found inducing a much higher titer of enzyme-linked immunosorbent assay antibody response but less protective than the inactived V263/TW02 vaccine did. The V263/TW02-VP2-C2 subunit protein might also be good for preparing serological diagnostic kits. The immunogenicity of V263/TW02-VP2-A3 subunit protein need to be further evaluated.
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