| Summary: | 碩士 === 國防醫學院 === 生理學研究所 === 91 === Nitric oxide (NO) has been implicated in immune--mediated neurotoxicity in neuron-glia cultures and with various inflammation-related diseases in the CNS. When glial cells are exposed to endotoxins, such as the bacteria endotoxin lipopolysaccharide (LPS), it produced different inflammatory modulators, including NO and cytokines.This study aimed to elucidate the roles and mechanisms of neurons in modulating the production of NO in glial cells stimulated by LPS. Neurons with mixed glia showed reduced LPS-stimulated inducible NOS (iNOS) expression and nitrite production compared to mixed glia alone; Howerver, neurons developed from neural progenitor for cells would not do so.This results suggested that the influence of neurons on glial activation may not be attributed to the cell-cell interaction. Several neuron-secreted neurotransmitters can inhibit the release of NO in glia cells. we found that several neurotransmitters such as epinephrine and glutamate could partially inhibit LPS-induced iNOS expression and NO production,. Future studies are needed to uncover the mechanisms of this neuron-induced inhibition. Since cerebral inflammation is important in many neurological disorders, this study might provide insight about the role of neuron-glia interactions in inflammatory responses which may be fundamental to clinical therapy neurodegenerative disease.
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