| Summary: | 碩士 === 國立嘉義大學 === 農學研究所 === 93 === Flower stalks of Cymbidium ensifolium var. misericors sterilized with 0.6%, 0.9% and 1.2% NaOCl 20 minutes and cultured with 1/3MS medium. It showed that upper flower stalks were not browning and survival rate was 100%. And that lower flower stalks were got serious browning and survival rate was only 20%. Whatever, it could get highest survival rate of flower stalks by 0.6% NaOCl sterilized 20 minutes. And took another sterilized pathway was flower stalks sterilized with 50、100、200mg/L Streptomycin sulfate mixed 50、100、200mg/L Gentamycin sulfate about 20minutes and 60minutes, after that, sterilized with 0.6% NaOCl 20 minutes again. It showed that the upper flower stalks sterilized with 50、100、200mg/L Streptomycin sulfate mixed 50 mg/L Gentamycin sulfate about 20minutes, the survival rate was 100%. The lower flower stalks sterilized with 200mg/L Streptomycin sulfate mixed 200mg/L Gentamycin sulfate about 60 minutes, the survival rat was 93%.
The rhizome of Cymbidium ensifolium var. misericors were cultured on MS medium with different salt concentration and pH value. It showed that the pH value were reduced, when medium were autoclaved. The pH value of 1/3MS, MS and 2MS medium with pH 6.0 and pH5.5 reduced than the other treatments significantly. When rhizome cultured 5 weeks, 1/3MS, pH value of MS and 2MS medium were about 4.03-4.23. And the pH value of 1/3MS, MS and 2MS medium with pH 6.0 and pH 5.5 reduced than the other treatments significantly that compared with per-autoclaved. The pH value of 1/3MS, MS and 2MS medium with pH 4.0 were increased. Followed the culture time, the growth of rhizome that cultured on MS medium with different salt concentration and pH value were increased than before cultured. Until 5th week, the length of rhizome was not different significantly between each pH treatment. And the length of rhizome cultured on 1/3MS was 0.69-0.77 cm longer than the other treatments. Whatever, 1/3MS (pH5.5) was the optimal medium for Cymbidium ensifolium var. misericors rhizome culture.
Flower stalks of Cymbidium ensifolium var. misericors cultured on 1/3MS medium contain different concentration of 2,4-D mixed with TDZ and NAA mixed with BA. It showed that the morphogenesis of stalks cultured on 1/3MS medium contain 2,4-D mixed with TDZ were higher than 1/3MS medium contain NAA mixed with BA. And the morphogenesis of upper flower stalks were higher than lower flower stalks. The browning was 0% and morphogenesis was 100% of upper flower stalks cultured on 1/3MS medium contain 0.5, 2.0 and 4.0mg/L 2,4-D mixed with TDZ. And followed increasing of TDZ concentration, number of shoots differentiation increase significantly. When TDZ concentration was more than 0.5mg/L, the shoot differentiation was reduced. When 2,4-D and TDZ concentration were increased, the number of rhizome differentiation was increased significantly. But the concentration of 2,4-D and TDZ were higher than 2.0mg/L and 1.0mg/L, rhizome differentiation were reduced. Rising TDZ concentration in lower 2,4-D concentration treatment, it would increase number of inflorcesease differentiation. But 2,4-D concentration was rise, the number of inflorcesease differentiation was reduced. Increased 2,4-D concentration, the flowering number was added and it would reduced significantly at 4.0mg/L 2,4-D treatment. The shoot number of rhizome was differentiated in 1/3MS contained with 1mg/L TDZ and 1mg/L 2,4-D more than the other treatment.
The respiration rate of Cymbidium ensifolium var. misericors rhizome cultured on MS medium in the tube was 150.3 mL CO2/kg‧hr at 5th day. After that, the respiration rate reduced significantly. The ethylene production of rhizome cultured on 1/3MS medium in the tube was 0.3μL C2H4/kg‧hr more than the other treatment at 7th day. The respiration rate of rhizome cultured on 2MS medium in the bottle was 317.5 mL CO2/kg‧hr at 3rd week. And the respiration rate of rhizome cultured on 1/3MS(T+D) medium in the bottle was 303.60 mL CO2/kg‧hr at 9th day. The ethylene production of rhizome cultured on 1/3MS(T+D) medium in the bottle was lower than the other treatment at 1st week, and highest at 3rd week (6.3μL C2H4/kg‧hr). After that, the ethylene production reduced significantly. The rhizome fresh weight was 1079.47 mg of 1/3MS medium higher than the other treatment significantly. The rhizome length was 4.19 cm and 4.05 cm of 1/3MS and 1/3MS(T+D) medium higher than the other treatment significantly. The number of shoot differentiation was 3.80 shoots and 4.27 shoots of 1/3MS and 1/3MS(T+D) medium higher than the other treatment significantly. The number of rhizome differentiation was 1.93, 2.13 and 2.53 of 1/3MS, 1/3MS(T+D) and MS medium higher than 2MS medium significantly.
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