| Summary: | 碩士 === 國立嘉義大學 === 農學研究所 === 91 === Tuberose (Polianthes tuberosa L.), an Agavaceae family, is one of bulb flowers and native in Mexico. It is one of the most important cut flowers in tropical and subtropical areas. To establish an efficient plant regeneration system, this study is to investigate the effects of explants, plant growth regulators and light intensity on inducing callus, suspension culture and plant regeneration of P. tuberosa ‘Double’and P. tuberosa cv. Chia-Nong ‘Bright Jewel’(single, white）.
The explants of young-petal, pedicel and leaf of tuberose were used for callus induction on MS basal medium containing 2,4-D, NAA (0, 0.5, 1.0, 2.0, and 4.0 mg/L) and kinetin, BA, TDZ (0, 0.5, 1.0 and 2.0 mg/L). The results showed that the young-petal was better explant than pedicel and leaf for inducing embryonic callus. Both pedicel and leaf explants cultured on medium containing NAA and kinetin, BA, TDZ had higher rates of rooting. The explants of young-petal cultured on basal medium containing TDZ alone produced translucent shoots.
Embryogenic suspension started rapid cell proliferation at the 12th day, especially on the MS medium containing 2,4-D 4 mg/L and kinetin 1 mg/L. The embryogenic cell was round, small and with dense cytoplasm. Later the cells divided and formed green clumps and then formed roots under light.
To induce somatic embryo and plantlet regeneration, the embryogenic calli were cultured on the basal media containing either maltose or sucrose (5, 20 g/L), and half or full strength MS salts. The results showed that somatic embryo formation and plant regeneration were not observed on these media. The calli appeared browning or rooting. When embryogenic calli were cultured on MS medium containing TDZ 1.0 mg/L under low light intensity (4μmol m-2s-1), somatic embryo and secondary somatic embryo could be obtained. After 4 weeks the plantlet were regenerated from somatic embryo subcultured with the MS medium containing TDZ 0.01 mg/L, with the rate of 14%. When embryogenic calli were cultured on the Modified MS medium containing NAA 0.1mg/L and BA 4.5 mg/L under low light intensity, somatic embryogenesis, mature and regenerated plantlets could be obtained. The regenerated plantlets cultured on the 1/2 MS medium containing TDZ 0.01 mg/L proliferated multiple shoots.
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