On the Conformational Changein Human Serum Albumin UsingElectrochemical and Surface PlasmonResonance Techniques

• Main Authors: Cheng-Feng Wu, 吳政豐
• Other Authors: Shean-jen Chen
• Format: Others
• Language: en_US
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/09670029505673417033

碩士 === 國立中央大學 === 機械工程研究所 === 91 === The conformational change in human serum albumin (HSA) has been investigated using dc-cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR) technique in this work. A slice of glass was sputtered a thin film of gold (47 nm), undergone self-assembly of 2-aminoethanethiol (AET) in ethanol solution and coated with the HAS solution to act as a specimen for this study. A stream of dichromate (Cr2O72-) solution flowed on the immobilized HAS caused a bio-interaction between the HAS and Cr (VI) thus resulting conformational change of the HAS. SPR and electrochemical techniques were used in the following to monitor the in-situ conformational change of the HAS due to its bio-interaction with Cr (VI).1. Monitoring using SPR technique: The interfacial change resulted from conformational change of immobilized HAS caused by Cr (VI) could be detected using SPR through the change of resonance angle.2. Monitoring using electrochemical techniques: (1) Shift in the open circuit potential (OCP) of the system reflected the conformational change of the HAS resulted from its toxic reaction with Cr (VI) ion. (2) Presence of current peaks in cyclic voltammograms (CV) at certain potentials were responsible for specific toxic reactions between the HAS and Cr (VI) ion. (3) Analysis of the resistive and capacitive data of the EIS provided a check on the conformational change of the HAS arisen from its toxic reaction with Cr (VI) ion. In additional to monitor the toxic reaction between the HAS and the hexavalent chromium. The validity and sensitivity of the SPR and electrochemical signals are discussed. The surface roughness of the system was estimated by atomic force microscope (AFM). The conformational change of the HAS was confirmed via analysis of vibration mode from the FT-IR spectroscopy.