A predictive system for gene isoforms based on alternative splicing variants and signals

• Main Authors: Chih-Cheng Yu, 游志誠
• Other Authors: Jorng-Tzong Horng
• Format: Others
• Language: en_US
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/14830537046209823531

碩士 === 國立中央大學 === 資訊工程研究所 === 91 === Alignments among ESTs, mRNA, protein sequences, and genome sequences are major approaches to reveal the gene isoforms. However, some isoforms can not be revealed by the alignments, because the ESTs are incomplete. Fortunately, such isoforms may be obtained by a prediction tool. In this study, we built a system to detect splice sites in the genomic DNA and to generate all possible isoforms. To provide detailed view of the gene structures, we developed a database, namely ISOdb, which integrated the ProSplicer database to reveal the alternative splicing in human genome. By aligning ESTs, mRNA, and protein sequences to the genome sequences, we provide a web-based interface for users to retrieve all possible isoforms that include alignments of the ESTs and other information. http://140.115.50.96/isodb/ Keywords : isoform, alternative splicing(AS), splice sites