| Summary: | 碩士 === 國立成功大學 === 環境工程學系碩博士班 === 91 === In this study we used Ames TestTM system, which offers reduced variability among experiments, requires smaller quantities of test chemicals and eliminate interactive systematic effects, were used to assess the mixture acute toxicity and genotoxicity. Three types of commercially available incenses sticks, and several traffic sites in Tainan, including a tunnel for motorcycles, a tunnel for automobiles, and a parking lot for diesel buses, were selected for this study. The ambient air at these sites and the smoke from incence burning and were collected for by high-volume samplers (PS-1), from October 2002 to January 2003, to assess the toxicity and genotoxicity of airborne SVOCs.
The collected samples were ultrasonic-extracted using dichoromethane as the solvent, fractionated into particle-associated (SOF) phase and vapor-
phase (XOC). All extractions were then solvent exchanged into dimethyl sulfoxide (DMSO), a solvent vehicle for later toxicity assessment.
GC analyses indicated that the relative concentration levels of PAHs in the air pollution by mobile sources are: motorcycle＞automobile＞diesel for all XOC and SOF. There are no significant differences among three types of incense sticks.. The XOC’s have higher mass percentage (70∼90％) than the SOF in mobile sources and incenses, excepted for motocycle (45％).
Ames Test showed that both of XOC and SOF of motocycle have highest toxicity amoung mobile sources. After activation by S9, the toxicity of extracts from mobile sources were always higher. The SOF of extracts from incense smoke is the major contribution to the toxicity and in the presence of S9, toxicity were also well-correlated to dose-response.
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