The study of neovasculizationin a collagen scaffold

• Main Authors: Pey-Tzy Lin, 林佩姿
• Other Authors: Ling-Hui HUANG
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/64885073298629869843

碩士 === 國立成功大學 === 生物科技研究所碩博士班 === 91 === In light of the rapid development of tissue engineering, angiogenesis has become one of the major issues in tissue and organ repair. Since the development of new tissues requires blood vessels to transport nutrient and remove wastes, it was found recently that, some angiogenic factors are capable of facilitating angiogenesis and tissue rehabilitation. Thus, this research aims at the screening of adequate angiogenic factors, and their ability to promote angiogenesis was investigated in an in vitro cell culture system. The functions of 4 various angiogenic factors was also examined. For a three-dimensional culture system, porous collagen matrices previously developed in our laboratory were applied. It was found in this study that the adding of angiogenic factors under 2-D condition exhibited a significant effect on cell activities. Further, the concentration of angiogenic factors, the cell types, and various combinations of angiogenic factors were shown to affect the behavior of cell migration. Besides, a co-culture system of simultaneously cultivating endothelial cells and fibroblasts in porous collagen matrices were established to evaluate and compare the effect on cell proliferation and angiogenesis. It was found that the existence of angiogenic factors in porous collagen matrices did facilitate angiogenesis. The immunohistochemical staining was further carried out in this study to corroborate the tubular structure which was composed of cells, and basement membrane formation around these tubular structures.