The investigation of adsorption isothem model and binding specificity of bilirubin imprinted material

• Main Authors: You-Ming Nian, 粘友明
• Other Authors: Mei-Jywan Syu
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/63755592377631433241

碩士 === 國立成功大學 === 化學工程學系碩博士班 === 91 === Molecularly imprinted polymer (MIP) was prepared by the interaction between the analyte or the template molecule and the functional monomer. During the polymerization, the cross-linker was added to confine the template molecule. Later, the template bound within the MIP was extracted by the utilization of solvent, the cavities as the recognition sites were thus formed. In this way, the MIP being made can have superior affinity and specificity towards the analyte. In this research, bilirubin, being the index for jaundice, was used as the template molecule. Methacrylic acid (MAA) and ethyleneglycol dimethacrylate (EGDMA) were used as the functional monomer and cross-linker, respectively. 2,2’-azo-bisisobutyronitrile (AIBN) was added to initiate the thermal polymerization. The MIP thus prepared was then washed by EDTA/NaOH solution for the extraction of bilirubin template molecules. Specific surface area analysis (Brunauer-Emmett-Teller analyzer; BET) and scanning electron microscope (SEM) were used to measure MIP’s physical properties as well as to observe its surface morphology. How the EDTA/NaOH solution affected the stability of bilirubin was investigated. The binding specificities of the MIP in the single solution and binary mixture were compared and discussed. The adsorption isotherm as well as the kinetic model was established. The parameters in the model were obtained and compared. The influence of salt for the adsorption performance was also discussed. The binding specificity of the MIP in triple mixture solution was further investigated. In addition, the serum samples were prepared to establish the calibration of bilirubin concentration via the adsorption of the MIPs.