| Summary: | 碩士 === 國立中興大學 === 生命科學院碩士在職專班 === 91 === This experiment studied 3 varieties of grape plantlets (Italia, Red Prince and Seneca Seedless) cultivated in vitro. Different levels of sucrose concentration were accompanied with different temperatures and treated with various types of growth retardants. The goals of this experiment is to find ways to reduce growth rate of grape plantets in vitro. The effects on plant height and numbers of new leaves were surveyed in those treatments. At the same time, the photosynthetic rate, respiration rate and physiological change on tonicity were recorded.
The cultivated treatments included different sucrose concentrations（0%, 3%, 6%, 7.5% and 9%）and different temperature treatments(25℃, 20℃, 15℃ and 10℃). Italia and Red Prince cultivated with 3% sucrose concentration at 10℃ showed the reduced growth rate, high survival rate, high transplant growth rate, and increased plantlet propagation, hence the best economical efficiency for short- to medium-term conservation.
Three varieties of grape plantlet were under different treatments of growth retardants, SADH and CCC. The Italia and Seneca Seedless treated with 10 ppm CCC, as well as all three varieties treated with 600ppm SADH showed the best growth retardant result.
With the suitable choice of sucrose and growth retardant concentrations accompanied with cultivation temperature, one should be able to reach the goal of medium-term conservation of grape cultivated in vitro.
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