| Summary: | 碩士 === 國立中興大學 === 園藝學系 === 91 === Six cultivars of poinsettia (Euphorbia pulcherrima) ‘Yellow Ancestor’, ‘G´EF 86-17’, ‘Pepride’, ‘Nobel Star’, ‘Peter Star’ and ‘Red Splendor’ were studied, optimal micropropagated through nodal culture, and induced mutation through somatic embryogenesis treated by γ-ray or chemicals .
Under dark condition, internode of ‘Nobal Star’, ‘Peter Star’ and ‘Red Splendor’ cultured on the medium containing CPA 0.8mg/l induced more callus. Callus were cultured on the solid medium containing CPA 0.4mg/l proliferated more global embryos, as well as the medium containing agar at 9 g/l did. In addition, riboflavin at 5 or 10 mg/l enhanced somatic embryo maturity.
When ‘Nobel Star’ irradiated by γ-ray, the survival rate was low. When callus of ‘Peter Star’ irradiated by 2Gy for once resulted the highest survival rate, as well as for twice developed more embryos. ‘Red Splendor’ callus had more survival rate when irradiated with 2Gy for twice.
Shoot tip of ‘Peter Star’ dipped NaN3 before culture, when the concentration or dipping duration increased, the callus formation decreased. The shoot tip treated with NaN3 at 0.5 or 2 μM for 2 hr, regenerated more somatic embryo.All shoot tips of ‘Yellow Ancestor’ treated with NaN3 0.5~2 μM for 1~4hr, before culture, the surrival rate were over 50﹪.
The single node of ‘G×EF 86-17’ was cultured in medium contain NAA 0~0.25mg/l combined BA 1~2mg/l had best growth. Nodal explants from lower position had better growth than from higher position, but the difference did not occur on ‘Yellow Ancestor’. ‘Pepride’ and ‘Red Splendor’ on medium contained with kinetin had longer but fewer shoots than that with BA. Also, additional riboflavin at 10-40mg/l decreased shoots from explant. ‘Pepride’ and ‘Red Splendor’ cultured on medium with agar 6g/l, proliferated more shoots of longer and more nodes, stem. For ex vitro rooting, shoot longer than 1.0cm of ‘Pepride’ or ‘Red Splendor’as dusted with IBA 2000ppm or NAA 500ppm, respectively, developed more and longer roots.
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