Characterization of Uncoupling Effects in Bamboo Shoot Mitochondria

• Main Authors: Jie Long He, 何杰龍
• Other Authors: Lee Feng Chien
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/96293025249321468012

碩士 === 國立中興大學 === 生命科學系 === 91 === Bamboo shoot mitochondria were isolated from Phyllostachys edulis and Bambusa oldhamii to study the uncoupling effect of fatty acids and respiratory inhibitors on mitochondrial. This study characterized the phosphorylation and non-phosphorylation respirations. The respiratory control ratio (RC ratio) was determined to be an index of how coupled the mitochondria are. In P. edulis mitochondria, the RC ratios were 1.12 and 1.03 in the presence of linoleic acid (LA) and palmitic acid (PA) as compared to the control of 1.38. With addition of oligomycin, the non-phosphorylation respiration rate was decreased from 18.71 to 15.02 nmol O2 mg min-1 protein-1. With addition of FCCP, the non-phosphorylation respiration rate increased from 18.01 to 31.68 nmol O2 mg min-1 protein-1. However, the non-phosphorylation respiration rate did not significantly change when SHAM, inhibiting alternative oxidase (AOX), was added. In B. oldhamii mitochondria, the RC ratios were 1.18 and 1.12 in the presence of LA and PA as compared to the control of 1.08. There was no significant change in non-phosphorylation respiration with addition of respiratory inhibitors. In P. edulis and B. oldhamii mitochondria, isolated with BSA, the respiration rates were both higher than without BSA. With addition of BSA in the reaction buffer, the uncoupling effect of LA and PA on non-phosphorylation respiration rate of P. edulis mitochondria can be inhibited. However, there was no significant change in B. oldhamii mitochondria.