Hot Pressurized Fluids Extraction of Gypenosides from Gynostemma Pentaphyllum

• Main Authors: Chun-Hao Chen, 陳君豪
• Other Authors: Chiehming J. Chang
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/00194569645675871688

碩士 === 國立中興大學 === 化學工程學系 === 91 === This study investigated the feasibility of extracting total gypenosides (TGP) from Gynostemma pentaphyllum (GP) by using batch and semi-continuous hot pressurized fluids extractions. Extraction conditions ranged from 50 to 1000 psig, 333 to 433 K, 0.5 to 3hr, 5 to 10mL/min, addition of 0% to 95% ethanol, and solvent to solid ratio using 50g GP/L~200g GP/L. Agitation speed was fixed at 200rpm to free of mass transfer resistance. Experimental results indicated that hot water under suitable pressures (i.e. 50 psig) enhanced the extracted amount of gypenosides, but temperature was the major effect on extracting gypenosides from GP. When semi-continuous hot pressurized water extracted GP at 200 psig, 373 K, 10 mL/min, 3 hours, and the ratio of 200g /L (GP/ Extractor Volume), the recovery of the gypenosides(106.7 mg/g GP) was 65.1 % of that obtained by using EtOH Soxhlet extraction for 24 (Check!!! ) hours. However, a semi-continuous 60% ethanol extraction of GP obtains the 100% recovery of total gypenosides. Finally, absorption purification using Amberlite XAD7-HP resin revealed that 96.1% recovery, 86.5% purity and 82.7%, 83.2% of solids containing TGP, could be obtained respectively for the pressurized water extract and the 60% ethanol extract. The enhancement of the purification was over three times.