Interaction of lead and gene polymorphism on renal function and blood pressure in long-term lead exposed workers.

• Main Authors: Chien-Wen Sun, 孫千雯
• Other Authors: Chi-Kung Ho
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/94qq2x

碩士 === 高雄醫學大學 === 職業安全衛生研究所 === 91 === We followed up a group of lead workers (n=530) from 1997 to 2002. The health examination data consisted of: blood lead levels, serum creatinine and uric acid levels, blood pressure levels. A questionnaire was used to collect potential confounder in the health examination. DNA was isolated from the whole blood of each worker. The ACE I/D and a-adducin G460W polymorphism are investigated by polymerase chain reaction and restriction fragment length polymorphism amplification of genomic DNA to define the The ACE I/D and a-adducin G460W genotypes. The main purpose of this study is to investigate the association among the different ACE I/D and a-adducin G460W genotypes to the renal functions and blood pressure in long-term lead exposed workers. The interaction of lead and gene polymorphism on renal functions and blood pressure was also evaluated in this study. In 2002, totally 530 lead workers were recruited. Of them, 312（59%）were men. The average age was 43（SD=7.9）years, and , mean working duration was 13（SD=7.8）years. Among them, blood pressure（mean systolic pressure: 123 mmHg; mean diastolic pressure: 77 mmHg）, renal function tests（mean creatinine: 0.95 mg/dL）, uric acid（mean: 6.37 mg/dL）were found. Their average blood lead level was 22 mg/dL. ACE I/D genotypes distributed as II: 192（42%） people, DI: 116（26%） people, II: 144（32%） people. a-adducin G460W genotypes distributed as GG: 124（28%）people, GW: 235（53%）people, WW: 88（19%）people. Regarding renal functions using general linear mixed models analyses with adjustment of age, gender, body mass index, smoking habit, we found that the interaction of blood lead levels and ACE I/D types associated to serum creatinine levels and creatinine clearance rate. The analyses of uric acid levels with general linear mixed models found that blood lead levels significantly related with serum uric acid levels after adjusting the confounders. For blood pressures by general linear mixed models analyses with adjustment of age, gender, body mass index, smoking habit, there was no relation between blood pressure levels and ACE I/D, a-adducin G460W genotypes, blood lead levels. In conclusion, the toxicity of lead to renal function may be affected by differential ACE I/D genotypes. Further studies and further assessment are clearly needed.