1、毛細管電泳法對強心配醣體藥物digoxin與digitoxin之同時分析研究2、毛細管電泳法對人體腦脊髓液和血漿中抗生素cephalosporins第四代藥物cefepime定量分析研究
碩士 === 高雄醫學大學 === 藥學研究所 === 91 === The purpose of this work is directed to the development of simple and sensitivity methods for the different medicines by micellar electrokinetic hromatography. The main study included simultaneous determination of cardiac glycosides and trace analysis of...
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Others |
| Language: | zh-TW |
| Published: |
2003
|
| Online Access: | http://ndltd.ncl.edu.tw/handle/97504667615666405063 |
| Summary: | 碩士 === 高雄醫學大學 === 藥學研究所 === 91 === The purpose of this work is directed to the development of simple and sensitivity methods for the different medicines by micellar electrokinetic hromatography. The main study included simultaneous determination of cardiac glycosides and trace analysis of cefepime in human cerebral spinal fluid and plasma by micellar electrokinetic chromatography. The results are summarized as follow：
1. Simultaneous Determination of Digoxin and Digitoxin by Micellar Electrokinetic Chromatography
A simple micellar electrokinetic chromatography is described for simultaneous determination of digoxin and digitoxin. The simultaneous analysis of digoxin and digitoxin was performed in Tris buffer (10 mM; pH 9) with 90 mM sodium dodecyl sulfate and 10% isopropyl alcohol as an anionic surfactant and organic modifier, respectively. Several parameters affecting the separation of the drugs were studied, including the pH and concentrations of the Tris buffer and sodium dodecyl sulfate. Partial application of the proposed method to the determination of digoxin in commercial tablets and in injections proved to be feasible.
2. Quantitative Determination of Cefepime in Human Cerebral Spinal Fluid and Plasma by Micellar Electrokinetic Chromatography
A simple micellar electrokinetic chromatography is described for determination of cefepime in human cerebral spinal fluid and plasma. The analysis of cefepime in human cerebral spinal fluid was performed in Tris buffer (20 mM; pH 9) with 200 mM sodium dodecyl sulfate as an anionic surfactant. The analysis of cefepime in in human plasma was performed in Tris buffer (10 mM; pH 8) with 150 mM sodium dodecyl sulfate as an anionic surfactant. Several parameters affecting the separation of the drugs were studied, including the pH and concentrations of the Tris buffer and sodium dodecyl sulfate. Application of the method to the determination of cefepime in human cerebral spinal fluid and plasma proved to be feasible.
|
|---|