| Summary: | 博士 === 高雄醫學大學 === 醫學研究所 === 91 === The analytical methods of malondialdehyde (MDA) and thiobarbituric acid reactive substances (TBARS) as biomarker for oxidative stress have been developed in the present study. The performance characteristics (linearity, detection limit, spike recovery, precision and accuracy) of the methods were carefully validated. The methods for the determination of MDA and TBARS are based on its reaction with thiobarbituric acid. The proposed methods were then applied to monitor the lipid peroxidation in in vitro study and the lipid peroxides in human hair. Three major works were carried out in this study. Firstly, a simple, continuous and automatic method was developed for on-line monitoring MDA as its TBA complex. This method involved the use of microdialysis sampling, on-line derivatization and on-line high performance liquid chromatography (HPLC) analysis. This method was applied to monitor the MDA-TBA adducts levels after treatment with H2O2 and metals to linoleic acid. In subsequent part, a sensitive and simple method for the determination of TBARS in hair has been developed. Alkaline hair hydrolysis method combined with simple spectrometric method has been employed to assess the degree of TBARS in hair. The method was then applied to the measurement of TBARS in hair of smokers and non-smokers. Finally, by combining microdialysis sampling with a HPLC separation technique, the analytical method for the determination of MDA in hair has been developed. These analytical methods proposed in the present study may benefit the further researches on the roles played by oxidative stress in biomedical science.
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