Study on the Hyphenated System of On-Line Microdialysis Sampling Coupled with Atomic Absorption Spectrometry and Its Biomedical Applications

• Main Authors: Tseng, Wei-Chang, 曾維昌
• Other Authors: Huang, Yeou-Lih
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/79315188468204721078

博士 === 高雄醫學大學 === 醫學研究所 === 91 === The hyphenated techniques of microdialysis sampling (MDS) coupled on-line with flow injection (FI) atomic absorption spectrometry (AAS) have been developed in the present study. The performance characteristics (linearity, detection limit, spiked recovery, precision and accuracy) of the on-line MDS coupled with AAS system were carefully validated. The determination and speciation of trace elements in in vivo living animals or in vitro biological fluids were performed by the proposed on-line MDS-FI-AAS system. Three major works were carried out in this study. Firstly, an on-line and fully automated MDS-HPLC-FI-HGAAS system has been developed to determine four urinary arsenic species [arsenite (AsIII), arsenate (AsV), monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA)]. The continuous and dynamic in vivo monitoring of four urinary arsenic species in bladders of anesthetized living rats were investigated using the proposed on-line method. In the subsequent part, an on-line MDS coupled with electrothermal atomic absorption spectrometry (ETAAS) has been developed by a home-made FI interface. The use of an on-line MDS-FI-ETAAS system permitted the in situ, dynamic and continuous in vivo monitoring of extracellular manganese in brains of anaesthetized living rats. Finally, a direct and rapid determination of diffusible calcium in human whole blood using on-line MDS coupled with FI flame atomic absorption spectrometry (FAAS) has been developed. The use of an on-line MDS-FI-FAAS system makes it possible to rapidly determine diffusible calcium in complex matrix of human whole blood without sample pretreatment. These on-line MDS-FI-AAS methods proposed in the present study may benefit the further researches into the biomedical mechanisms of trace elements.