| Summary: | 碩士 === 中原大學 === 醫學工程研究所 === 91 === Pulsed Electromagnetic Fields（PEMF） has many medical applications. Previous animal and clinical studies have clearly showed a positive effect of PEMF on the rate of osseous repair. The purpose of this study is to find the optimal parameters of PEMF stimulation on the growth of osteoblasts.
The cranium off new born Wistar rats were harvested and cultured. The experiment were divided into two parts : The first part was to find the better exposure time of the single pulse ( 7.5 Hz ) electromagnetic field with induced electric field intensities of 2 mV/cm, which investigated in our previous studies. A PEMF stimulation was applied to osteoblasts for three different stimulation periods of 1 hour , 2 hours, and 4 hours per day, and 3 stimulation day : 1 day, 2 days, and 5 days.
In the second part, we used the better stimulation time in part one of 1.5, 7.5, 10 or 75 Hz on osteoblasts proliferation promotion to find the optimal frequencies.
After the stimulation, the alkaline phosphatase ( ALP ) stain would be used for osteoblast cell activity, and the osteoblast cells proliferation were analyzed by the 3-〔4, 5-dimethylthiazol-2-yl〕-2,5-diphenyltetrazolium bromide (MTT) assay.
The results showed that the PEMF could enhance the osteoblast cells proliferation when the stimulation time was 4 hrs/day, and 5 days exposure. The frequency of 1.5Hz, 7.5Hz, or 10Hz could stimulate the osteoblasts proliferation, but 1.5 Hz was more significantly than others.
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