The Phylogenetic analysis of Rotaviruses in the Central Taiwan

• Main Authors: Hsin-Chuan Lai, 賴信全
• Other Authors: Chi-Chiang Yang, Ph.D.
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/42349158310844300855

碩士 === 中山醫學大學 === 醫學研究所 === 91 === Rotaviruses are a major cause of gastroenteritis in children worldwide. Phylogenetic analysis of the partial VP7 gene of the human rotavirus in central Taiwan. Total 81 stool samples from cases of acute gastroenteritis, occurring in the hospital of the central Taiwan at 1996, 2002, 2003, were selected by polyacrylamide gel electrophoresis and reverse transcription-polymerase chain reaction (RT-PCR) for partial VP7 gene of the human rotavirus group A, B, C. Then confirmed by comparative nucleotide and deduced amino acid sequence analysis for the relative sequence. A total of 10 stool sample were selected by polyacrylamide gel electrophoresis. The sequence analysis of the gene encoding the major neutralization glycoprotein (VP7) was performed on ten human isolates of serotype 1,3,9 of rotavirus and their genetic variations were examined. The partial VP7 gene of nucleotide sequence and the deduced amino acid sequence of CS96-40 of G1 are similar to MVD9816 (identity rate 97.15%, from Uruquay)；The sequence of CS02-01 of G3 are similar to 98-B31 (identity rate 98.93%, from Japan)；The sequence of CS01-05, CS01-06, CS01-07, CS01-09, CS01-13, CS02-02, CS02-03, CS02-04 and established G9 rotaviruses sequence are very similar (identity rate 96.85% ~ 99.88%), especially CS02-04 and SP2737 (from Japan) the identity rate is 99.88%. The first data of the human rotavirus group A serotype G3, G9 distribution in central Taiwan is reported. These data are important for the development of an appropriate rotavirus vaccine.