| Summary: | 碩士 === 中山醫學大學 === 毒理學研究所 === 91 === Abstract
Oral cancer is one of the ten leading causes of cancer death in Taiwan. According to Health and Vital Statistics Republic of China in 2002, oral cancer is the fifth leading cause of cancer death in man. The mortality rate of oral cancer was 13.09 per 100,000 and gradually increasing. Epidemiological studies have indicated that the cigarette smoking, alcohol drinking and betel quid chewing are the major risk factors of oral cancer in Taiwan. In the present study, we use loss of heterozygosity (LOH) and microsatellite instability (MIN) as the index to explore the features of genomic instability of male oral squamous cell carcinoma (OSCC).
With Laser Capture Microdissection (LCM) and 382 fluorescence labeled microsatellite markers, we have analyzed 30 male OSCC tumors for the loss of heterozygosity and microsatellite instability. The chromosome arms with high LOH frequency were 1q、2q、3p、4p、4q、5p、5q、6q、8p、8q、12p、14q、17p and 19p. Twenty-six markers on 16 chromosome arms showed high LOH frequency (>40%). The top 10 markers with high LOH frequency were D10S537 (62.5%), D17S799 (61.5%), D14S258 (60%), D7S640 (58.3%), D19S216 (57.1%), D9S287 (54.6%), D1S2868 (50%), D1S2836 (50%), D4S415 (50%) and D6S1581 (50%) in order. Twenty-three chromosome regions, mainly in the loci of tumor suppressor genes and DNA repair genes demonstrated high LOH frequency (> 46%). Furthermore, there were 5 and 4 LOH specific markers for tongue (D3S1311, D5S2027, D8S258, D12S368 and D14S985) and buccal cancer (D1S2878, D1S199, D5S424, and D6S308), respectively. The frequency of MIN OSCC tumor was high in Taiwan (63.3%). Specific high frequency of microsatellite instability (78.6%) for tongue cancer was found on chromosome arm 11q. There were 2 and 6 microsatellite instability specific markers for tongue (D5S424 and D11S925) and buccal cancer (D1S452, D3S1289, D6S422, D7S507, D9S171 and D20S115), respectively. Taken together, high frequency of genomic instability was observed in Taiwanese male OSCC tumors and distinct markers for tongue and buccal cancer were found respectively.
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