Study on the Use of Norepinephrine Nanoparticle Preparation for Transdermal Absorption

• Main Authors: Chiun-Chieh Wang, 王俊傑
• Other Authors: Hung-Hong Lin
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/83648042909159859574

碩士 === 嘉南藥理科技大學 === 生物科技系暨研究所 === 91 === This study described the preparation and characterization of hydrogel nanoparticles containing the neurochemical messenger norepinephrine (NE). NE as a model drug was encapsulated in hydrogel nanospheres using homogenizer. The size of the NE-hydrogel nanoparticles was evaluated by using Zetasizer 3000HS particle size analyzer. The rheological properties of ointments including NE-hydrogel nanoparticles were characterized by Cone and Plate Viscometer. All drug-polymer interactions and the skins were assessed by Differential Scanning Calorimetry (DSC). For these nanoparticles, drug loading, in vitro release of drug from the nanoparticles, drug concentration after transdermal absorption , cumulative amount of drug in skin and long-term stability determination were quantified by high performance liquid chromatography (HPLC). The results revealed that particle size, encapsulation efficiency, in vitro release and transdermal absorption properties of the nanoparticles were affected by various hydrogels, preparative processes, surfactants and drug concentrations. The measured particle size distribution was ranged from 40nm to 500nm. The time-depentent behavior of ointments including NE-hydrogel nanoparticles was mentioned to as 〝Dilatant〞 flow and showed a shear-thickening liquid. In regard to the long-term stability, the results suggested that the product containing NE nanoparticles ought to be stored in the condition of lyophilized powder at lower temperature and recovered by adding double deionized water before used. The DSC determinations indicated that the physical and chemical interaction did not occur among the components during manufacturing process and reconstituted lyophilized powders. The encapsulation efficiency of NE in hydrogel nanoparticles was reached to 81. 32%. On the basis of in vitro release study, it revealed that NE was encapsulated in the hydrogel matrix, following the initial burst release of 30% to 40%, the release was sustained over the 24-hours study period. For　in vitro transdermal absorption study, the skins were treated as well as the ointments were added with and without different concentrations (0.5% and 1%) of various surfactants (benzalkonium chloride, sodium lauryl sulfate and tween 80), the experimental results revealed that the rapid permeability was correlated with surfactants and drug concentration, the skin barrier property was significantly compromised with benzalkonium chloride and　sodium lauryl sulfate treatment, tween 80 had a lesser effect on skin barrier property compared to benzalkonium chloride and　sodium lauryl sulfate (　1% > 0.5%, benzalkonium chloride > sodium lauryl sulfate > tween 80).