The inhibition activity of Chinese herbs extracts on UVB induced cell death

• Main Authors: Chia-Ju Tsai, 蔡佳儒
• Other Authors: Ching-Gong Lin
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/59400648496109337422

碩士 === 嘉南藥理科技大學 === 生物科技系暨研究所 === 91 === The object of this research is use microwave to extract Chinese herbs, and investigate activity inhibition of Chinese herbs extraction on UVB induced cell death. B16 melanoma cell is introduced for UVB expose with several Chinese herbs extractions, such as licorice root, mulberry, white mulberry, ginkgo, angelica root, Paeony root bark. Especially “licorice root” shows better inhibition result. Thus, we chose the follow four major compounds of licorice root to do the next experiment, such as Glycyrrhizic acid (GZA), 18? Glycyrrhetinic acid, 18β-Glycyrrhetinic acid (GTA), dibenzoylmethane(DBM). Except DBM, the other shows over 50~70% cell viability in different concentrations. In additional, using gel electrophoresis to observe the change of DNA with Chinese herbs extraction. And DAPI is also observed fluorescence due to change of nuclei. Another side, to investigate relation between cell activity and protein synthesize, we use cycloheximide to analysis, which is a kind of protein neosynthesis inhibitor. From the experiment result, we know that cell death inhibition of Chinese herbs is relative to protein synthesize. Besides, for study inhibition of Chinese herbs extraction whether is relative to scavenge of free radical. Using　 L-(+)-Ascorbic acid, (-)-epigallocatechin gallate (EGCG) with damaged cell by UVB. Both of them are no influence to the cell, so infer that inhibition is not relative to scavenge of free radical. At the final, apply Chinese herbs with sunscreen (SPF4), within Chinese herbs extraction will gets more protection result than without. This result could do advanced application on therapy of skin, and make UVB damaged skin get improve.