Effect of Drinking Habits and CYP2E1 and ALDH2 Gene Polymorphisms on Hepatic Function

• Main Authors: Zhi-Wei,Wang, 王志偉
• Other Authors: 吳芳鴦
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/20424956247624911720

碩士 === 中國醫藥學院 === 環境醫學研究所 === 91 === Abstract According to study results, drinking popularity of the aborigines was higher than non-aborigines. This tendency has been increased by 10 times for the past thirty years. As health problems resulted from wine drinking have always been a pain for aboriginal tribes, it is generally considered that such drinking habits are related to heredity. This research was based on the questionnaire surveys and blood samples collected from complex checkups conducted in He Ping Country in 2001 for an exploration of the relationships among drinking habits, basic characteristics of individuals, medical history and alcohol metabolism genes (ALDH2 and CYP2E1). In addition, the impact upon liver functions imposed by the correlation between drinking habits and alcohol metabolism genes was also discussed. Among the examinees who participated in complex checkups in He Ping Country in 2001, 499 people were drinking for the past half year. Three groups were divided according to drinking habits. There were 289 people in Group I who drank for 1 to 2 days per week. There were 110 people in Group II who drank for 3 to 4 days a week and Group III had 92 people who drank every day. The examinees were asked for their agreement and signature in advance for questionnaire surveys and blood drawing for genotype analysis. For ALDH2 and CYP2E1 genotype tests, individual product after increase of polymerase chain reaction (PCR) was cut by restriction enzymes Mbo II and Pst I and different fragments were separated via electrophoresis, which resulted in the genotype of the gene. The results showed that frequency of ALDH2*1/*1 genotype was 83.2%, that of ALDH2*1/*2 genotype was 16.2%, and that of ALDH2*2/*2 genotype was 0.6%. For CYP2E1 genotype, frequency of c1/c1 type was 70.6%, that of c1/c2 type was 24.2% and that of c2/c2 type was 5.2%. After division of races, it was found that the distribution of ALDH2 was different between aborigines and non-aborigines. Non-aborigines with allele ALDH2*2 were more than aborigines; however there was no significant difference of CYP2E1 genetic distribution between aborigines and non-aborigines. For drinking habits, aborigines had a higher rate (28.4% vs. 22.1%) and female aborigine drinkers were 2.3 times more than female non-aborigines. If the drinkers had allele ALDH2*1, they took a higher risk of abnormal liver functions than the drinkers with allele ALDH2*2 by 2.28 times. Daily drinkers with ALDH2*1 took a even higher risk than the drinkers who drank for 1 to 2 days per week with allele ALDH2*2 by 3.4 times. There seemed no significant correlation between CYP2E1 and drinking habits or liver functions because no statistically significant difference was found. Conclusion: There is a tightly connected relationship between drinking habits and ALDH2*2 genotype, which becomes a potential protection factor for the drinkers in He Ping Country to prevent abnormal liver functions. However, a relevant relationship between CYP2E1 genotype and drinking habits and liver functions has not yet been found.