| Summary: | 博士 === 中國醫藥學院 === 中國醫學研究所 === 91 === THE STUDY OF ELECTROACUPUNCTURE AND NOS INHIBITOR
ON ANALGESIA- BY THE MODE OF FORMALIN STIMULATION
AND MEASUREMENT OF FOS PROTEIN IN WISTAR RATS
Yun-Tson Tsao
Institute of Chinese Medical Science, China Medical College, Taichung, Taiwan
Major Professor：Jaung-Geng Lin
Abstract
Systematically administrated nitric oxide synthase (NOS) inhibitor, L-Nω nitro-arginine methyl ester (L-NAME), does-dependently reduced c-fos expression in the rat lumbar spinal cord. The effects of treatment of NOS inhibitors include anti-inflammation in peripheral system and neruogenicity in central system. Dosage in the route of spinal cord could exclude the anti-inflammation effect of NOS inhibitors. To investigate the effects of activated NOS in spinal cord on neuronal activities and related behaviors of pain following peripheral stimulation. We examined formalin-induced hyperalgesia and c-fos expression following treatment of NOS inhibitor （ L-NAME ） by the route of spinal cord combined with electroacupuncture on Zusanli in order to seek the best anti-algesia strategy.
We observed that L-NAME (300, 1000 nM) administrated in the route of spinal cord combined with （10X,20X ）intensity of electroacupuncture on Zusanli decreased both primary and late stage of hyperalgesia effects induced by formalin ;And these treatments decreased the amount of c-fos-labeled neurons in all three regions of dorsal horn（ the superficial lamina, nucleus propius and deep lamina ） in a likely dose dependent manner. Electroacupuncture combined with L-NAME treatments maybe the best choice of the analgesia strategy in the clinics.
Key words: electroacupuncture, L-NAME, Fos, analgesia
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