Effects of N-acetylcysteine on enterovirus 71-infected human neural cells

• Main Authors: Ying-Chi Chao, 趙英淇
• Other Authors: Shin-Ru Shih
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/60075199916562826762

碩士 === 長庚大學 === 基礎醫學研究所 === 91 === Recent studies showed that oxidative stress might play a role in virus infection, however, less is known about the relationship between enterovirus 71 infection and oxidative stress. Here we used the human neural glioblastoma SF268 cell line to investigate the effect of oxidative stress on EV71 infection. In this study, we showed that EV71 infection is accompanied by the generation of oxidative stress and the level of ROS is associated with the infection time and virus titer. We also found that viral replication and the generation of ROS were associated. In addition, an antioxidant, N-acetyl-L-cysteine (NAC), showed antiviral activity at low virus titer in use of plaque assay. NAC also inhibited EV71-induced apoptosis in SF268 cells at the first 24 hr, but these inhibition are limited to 30-40%. In the time course study, NAC exhibited better antiviral activity in the early stage of viral infection. Taken together, oxidative stress might be involve in the early stage of EV71 infection and the NAC might have the potential to protect cell from virus-induced apoptosis.