奈米結構材料於毛細管電泳之應用

• Main Author: 林泰安
• Other Authors: 周禮君
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/05761838349875158203

碩士 === 國立中正大學 === 化學研究所 === 91 === Abstract In this research, novel nanostructured materials were prepared and applied to capillary electrophoresis for separation (part 1) and chromatographic detection (part 2). Part 1 of this thesis describes the sol-gel processing of organo- and bio-functionalized sol-gel materials by using sol-gel precursors that were functionalized with -N(CH3)3+ 、Pirkle type chiral center, and HSA. These sol-gel-derived nanocomposite have been filled into capillaries as monolithic stationary phases for capillary electrochromatographic (CEC) separation. Part 2 of this thesis describes the development of an unique indirect detection method by immobilizing a monolayer of colloidal silver on the inner capillary window. The feasibility of this detection mode has been demonstrated with sucrose as samples. When a sucrose sample flows through the monolayer surface of colloidal silver, it causes the spectral maximum of colloidal silver red shift. By detecting the spectral difference of colloidal silver, sucrose samples can be indirectly detected.