| Summary: | 碩士 === 國立陽明大學 === 環境衛生研究所 === 90 === Alkylphenols ( APs ) are compounds widely used as plastic, rubber additives and antioxidants and for surfactants. Alkylphenols are known estrogenic xenobiotics in fish, birds and mammals. Although some of the alkylphenols present in the environment are known to be released from plastic material, the main origin for this contaminant is from the microbial break-down of alkylphenols polyethoxylates ( APEOn ), a large group of nonionic surfactants. Nonylphenol ( NP ), octylphenol ( OP ) and butylphenol ( BP ) are the most common alkylphenols. Therefore, human usually expose to alkylphenols, compounds of endocrine disrupters.
Research on human biological samples of alkylphenols are rare, and the analytical reference method is few, either. Therefore, we have developed a method for simultaneous determination of NP, OP and BP in human urine. Urine samples were hydrolyzed with b-glucuronidase/ arylsulfatase and cleaned-up with Varian PH 500 mg/3 mL solid phase extraction cartridges. Subsequently, the samples were analyzed by using HPLC-fluorescence detection. Recoveries and CV value for urine samples were 83.64±7.50 % ( 7.04% ) for NP, 84.14 ±7.29% ( 7.76% ) for OP and 76.43±6.35% ( 7.51% ) for BP. Working standards, ranging from 5 mg/L to 0.5 mg/L, were prepared by diluting the stock solutions in methanol. The quantitative concentrations in urine were 1.60 mg/L for NP, 1.57 mg/L for OP and 1.64 mg/L for BP. We applied the established method to urine samples of the university students ( n=9 ), the housekeeping workers ( n=13 ) and the ABS plastic industry workers ( n=10 ). We find that the three groups are not high exposure groups of NP, OP and BP.
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