Kur-Ta Cheng

• Main Authors: Ming-Jing Yang, 楊旻璟
• Other Authors: 鄭可大
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/27239914669993537298

碩士 === 台北醫學院 === 醫學研究所 === 90 === Anoectochilus formosanus Blume, belonging to Orchidaceae, is a perennial herb. It is used as a medicinal plant in folk. There are four lines different lines within the species according to their appearance: red stem, green stem, narrow leaf, and round leaf. The molecular markers screened by fingerprinting techniques are taken as the detectable object to provide complete and abundant genetic information for the researchers. In rDNA sequence analysis, no significant variation was found among the four entried lines. In RAPD analysis, three primers, T12+A, T12+T and D1+G, generated polymorphic markers between the lines of red stem and green stem. However, six screened primers, 808, 827, 835, 836, 841, and 842, were able to completely differentiate the four lines when ISSR analysis was perfomed. In addition, five sets of AFLP primers were recommended in our study to detect the somatic variation among the excised shoots derived from the same individual. Our recent data shown that polysaccharide in A. formosanus exerts immune activity in vivo. No significant difference in total polysaccharide content was found among the four lines, but acidic and basic plysaccharide contents were different. The polysaccharide content along with RAPD markers and ISSR markers obtained in the study will be employed to set up a model for selection in breeding program.