Analysis of Anti-Heat Shock Protein 60, -70 and -90 Autoantibodies in Patients with Manic Episode

• Main Authors: Chia-Yi Lin, 林佳儀
• Other Authors: Sy-Jye Leu
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/09865207747787304007

碩士 === 台北醫學院 === 細胞及分子生物研究所 === 90 === A close interaction between the central nervous system and the immune system have evidenced in many documents, in which we have paid attention to the study of immunological abnormalities both in psychological stress and patients with psychotic disorder. More specifically, an autoimmune mechanism was suggested as one of the possible factors in the pathogenesis of schizophrenia. The aim of this study was to investigate the presence of antibodies that react with heat shock proteins (Hsps) in sera from patient with mania. The sera were tested for antibodies binding to cell extracts of U-373MG glioblastoma cell line using western blot analysis. Hsps are intracellular proteins in most cells which are thought to serve protective functions against infection and cellular stress. In some instances, Hsps may be aberrantly expressed on target tissues interaction with the immune system might potentially result in autoimmune disease, as suggested in juvenile diabetes mellitus or rheumatoid arthritis. In the part of autoantibodies against Hsp90, we observed that un-medicated acute patients were significantly higher compared with remission patients. Sera anti-Hsp90 autoantibodies in medicated acute patients were significantly lower compared with un-medication acute patients. Sera anti-Hsp 70 and 90 autoantibodies in medicated acute patients were significantly lower compared with normal controls. In addition, there was no significant difference on autoantibodies against Hsp60 between patients and normal controls . We suggested that a presence of abnormal immune reactivity involving Hsp90 in patients with manic acute episode, and it may implicate in pathogenesis in acute episode with this disease.