The Effects of various rice containing different amounts of amylose on lipid metabolism in STZ-induced diabetic rats in low dietary fiber level status

• Main Authors: Chia-Wen Chen, 陳嘉文
• Other Authors: Hsing-Hsien Cheng
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/13546730057860706525

碩士 === 台北醫學院 === 保健營養學研究所 === 90 === The purpose of this study was to investigate the effects of various rice containing different amounts of amylose on lipid metabolism in STZ-induced diabetic rats given a low-dietary-fiber diet. This study was divided into two experiments. Both experiments used 16-week old male Wistar normal or diabetic rats as experimental animals for 4 weeks of experimental dietary feeding. Diabetes was induced by nicotinamide and streptozotocin injection. The formulas of these experimental diets were modified from the AIN93M formula. In experiment I, the experimental rats were divided into two groups: a normal rat group (NC) and a diabetic rat group (DC), each group with ten rats. Both groups were fed the experimental diets with 5% dietary fiber and gelatinized cornstarch as the carbohydrate source. The purpose of experiment I was to investigate differences in lipid metabolism between normal rats and diabetic rats. In experiment II, the rats were divided into three groups and fed three diets Indica rice (DI), Japonica rice (DJ), and Waxy rice (DW), each group with ten diabetic rats. All groups were fed the experimental diets with 2.5% dietary fiber and gelatinized Indica rice, Japonica rice or waxy rice as the carbohydrate source. The purpose of experiment II was to investigate differences in lipid metabolism in diabetic rats fed the three diets with 2.5% dietary fiber. Fasting blood sampling and an intraperitoneal glucose intolerance test (IPGTT) were conducted in the first week initially and the fourth week in both experiments. At the end of both experiments, all rats were sacrificed, and their livers and epididymal fat pads were collected. Results of experiment I showed those diabetic rats did not have hypercholestinemia or hypertriglyceridemia, and the normal rats had significantly higher activity of fatty acid synthase in the liver than did diabetic rats. Results of experiment II showed that rats eating the DI and DJ diets had significantly higher plasma TG and VLDL-TG concentrations, than did those on the DW diet. Fatty acid synthase activities of the liver in the DI and DJ diets were significant higher than that of the DW diet. But rats on the DW diet had significantly higher activity of fatty acid synthase in epididymal fat pads. In conclusion, hypertriglyceridemia was induced in diabetic rats by feeding an experimental diet with 2.5% dietary fiber and higher amylose rice as the carbohydrate source.