| Summary: | 碩士 === 台北醫學院 === 保健營養學研究所 === 90 === Glucosinolate derivatives, phenyl ethyl isothiocyanate (PEITC), benzyl isothiocyanate (BITC) and indole-3-carbinol (I3C), are thought to be the bioactive components in cruciferous vegetables. A lot of studies have demonstrated that glucosinolate derivatives are effective inhibitors of tumorigenesis in induced animal models, and of the growth of many lines of cancer cells. Because lung cancer is the first leading cause of cancer, the aim of the present study was to determine whether PEITC, BITC and I3C inhibit the growth of human lung carcinoma A549cell line, and to investigate the molecular mechanisms of PEITC- and BITC-inducted apoptosis in A549 cells. The results showed that PEITC (5~25μM), BITC (2.5~25μM) and I3C (50~250μM) suppressed the growth and the viability of A549 cells in a dose-dependent manner (p< 0.05). Flow cytometric analysis indicated that treat with PEITC (5~25μM) and BITC (7.5~25μM) could increase the percentage of sub G0 cells (apoptotic cells) and G2/M cells in the cell cycle, although higher concentrations of PEITC and BITC (25μM) apparently had less apoptotic cells than the contration of 10μM. PEITC and BITC (10μM) increased the apoptotic cells for 24, 48 and 72 hrs by time dependent manner as well (p< 0.05). Compared with PEITC and BITC, fewer apoptotic cells and more G0/G1 cells were induced by 250μM I3C. Fluorescent microscopy showed that apoptosis was more pronomineat at 10μM of PEITC and BITC after 24 hrs treatment, but higher concentration (25μM) of PEITC and BITC induced cell nerosis rather than apoptosis. Western Blot analysis indicated that PEITC and BITC could upregulate the P53 and P21 protein level in dose-and time-dependent manner, but did not affact the Bax protein level. In conclusion,cruciferous vegetable derivatives, PEITC and BITC suppressed A549 cell growth in part by induction of apoptosis which associated with P53 and P21,but not Bax protein expression.I3C also suppressed A549 cell growth, but induction of apoptosis is not the major pathway.
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