| Summary: | 碩士 === 國立臺灣大學 === 流行病學研究所 === 90 === Enterovirus 71 (EV71) has been implicated as the etiological agent for children infection with neurologic complications, developing effective vaccines is considered a top choice among all control measures. In an EV71 vaccine study, the VP1 DNA vaccine was compared with the inactivated virus vaccine in its ability to elicit maternal antibody and to provide protection against lethal EV71 infection in suckling mice. Although both groups of vaccinated dams possessed similar levels of neutralization antibodies prior to gestation, mice that received inactivated virus vaccines mounted a higher total-IgG response and provided a greater efficacy in passive protection study than mice received VP1 DNA vaccines. Other than VP1 protein, the major viral neutralization determinant, the capsid of enterovirus is composed of three more structure proteins, VP2, VP3 and VP4, which might also be associated with antigenicity of the virion. Therefore, we constructed DNA vaccines composed of EV71 structural genes, VP1-VP4, and investigated comparatively whether these genes alone or in combination could induce protective immunity in ICR mice model. The aims of this work were to investigate whether VP2, VP3 or VP4 could help VP1 vaccine induce protective immunity and further find out the appropriate components of EV71 DNA vaccine.
The data showed that VP2 DNA vaccine seemed to have efficacy in eliciting protective immunity. In addition, we found that DNA vaccine composed of all four components seemed to stimulate a higher level of total IgG and provided better protection against lethal EV71 infection in ICR newborn mice model. Although our data did not achieve statistical significance and the efficacy of these DNA vaccine preparations containing different components should be investigated in a more careful manner, our data could be still helpful in future work of EV71 DNA vaccine development.
Keywords: Enterovirus 71; DNA vaccine; passive protection; animal model.
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