The Role of PML in T cell Activation and Apoptosis

碩士 === 國立臺灣大學 === 免疫學研究所 === 90 === Abstract Promyelocytic leukemia (PML) protein is found concentrated in nuclear body along with other proteins including pRB, P53, Daxx, CBP and SUMO-1. Various functions have been attributed to the PML nuclear body, but the exact biochemical...

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Main Authors: Wan-ni Yu, 兪宛妮
Other Authors: Ming-Zone Lai
Format: Others
Language:zh-TW
Published: 2002
Online Access:http://ndltd.ncl.edu.tw/handle/03996022729132045932
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spelling ndltd-TW-090NTU015430022015-10-13T14:41:12Z http://ndltd.ncl.edu.tw/handle/03996022729132045932 The Role of PML in T cell Activation and Apoptosis 探討PML在T細胞活化及凋亡上扮演的角色 Wan-ni Yu 兪宛妮 碩士 國立臺灣大學 免疫學研究所 90 Abstract Promyelocytic leukemia (PML) protein is found concentrated in nuclear body along with other proteins including pRB, P53, Daxx, CBP and SUMO-1. Various functions have been attributed to the PML nuclear body, but the exact biochemical mechanism is still unclear. PML is known to involve in cell growth, tumor suppression, apoptosis, and immune response. Here we investigated the role of PML on T cell development, T cell activation, and T cell apoptosis by transgenic expression of PML and PML△3K. We generated transgenic mice expressing PML or PML△3K under the control of the T cell-specific CD2 promoter. In PML transgenic mice, total thymocyte number and splenocyte number remained normal. The frequency of double-negative, double-positive and single-positive thymocytes was similar between PML transgenic and wild-type mice. In addition, within the double-negative thymocytes, the development of CD44+CD25-, CD44+CD25+, CD44-CD25+ and CD44-CD25- was normal. Positive selection was not affected in thymocytes of PML transgenic mice. Levels of TCR expression were also indistinquishable between wild-type and PML transgenic mice. There was an increase in splenic CD4+ population of the PML transgenic mice, yet TCR expression on splenocytes was identical between PML transgenic and wild-type mice. Thymocytes from PML transgenic mice were equally sensitive to Fas-induced apoptosis as wild-type. The proliferation of the PML transgenic thymocytes and splenic T cell were normal. For PML△3K transgenic mice, total thymocyte number and splenocyte number remained normal. PML△3K transgenic mice were normal in thymocyte subsets, thymic positive selection, splenocyte subsets, and sensitivity to Fas-induced cell death. The only defect observed within PML△3K transgenic mice was reduced proliferation in thymocytes. The proliferation of PML△3K splenic T cell, however, was normal. Our results suggest that endogenous level of PML is sufficient to maintain normal T cell development and activation, which is mostly unaffected by PML overexpression. In contrast, proper sumoylation of PML is likely essential for T cell activation, inhibit of PML sumoylation ( and nuclear body localization ) interfered with T cell proliferation. Ming-Zone Lai 賴明宗 2002 學位論文 ; thesis 59 zh-TW
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description 碩士 === 國立臺灣大學 === 免疫學研究所 === 90 === Abstract Promyelocytic leukemia (PML) protein is found concentrated in nuclear body along with other proteins including pRB, P53, Daxx, CBP and SUMO-1. Various functions have been attributed to the PML nuclear body, but the exact biochemical mechanism is still unclear. PML is known to involve in cell growth, tumor suppression, apoptosis, and immune response. Here we investigated the role of PML on T cell development, T cell activation, and T cell apoptosis by transgenic expression of PML and PML△3K. We generated transgenic mice expressing PML or PML△3K under the control of the T cell-specific CD2 promoter. In PML transgenic mice, total thymocyte number and splenocyte number remained normal. The frequency of double-negative, double-positive and single-positive thymocytes was similar between PML transgenic and wild-type mice. In addition, within the double-negative thymocytes, the development of CD44+CD25-, CD44+CD25+, CD44-CD25+ and CD44-CD25- was normal. Positive selection was not affected in thymocytes of PML transgenic mice. Levels of TCR expression were also indistinquishable between wild-type and PML transgenic mice. There was an increase in splenic CD4+ population of the PML transgenic mice, yet TCR expression on splenocytes was identical between PML transgenic and wild-type mice. Thymocytes from PML transgenic mice were equally sensitive to Fas-induced apoptosis as wild-type. The proliferation of the PML transgenic thymocytes and splenic T cell were normal. For PML△3K transgenic mice, total thymocyte number and splenocyte number remained normal. PML△3K transgenic mice were normal in thymocyte subsets, thymic positive selection, splenocyte subsets, and sensitivity to Fas-induced cell death. The only defect observed within PML△3K transgenic mice was reduced proliferation in thymocytes. The proliferation of PML△3K splenic T cell, however, was normal. Our results suggest that endogenous level of PML is sufficient to maintain normal T cell development and activation, which is mostly unaffected by PML overexpression. In contrast, proper sumoylation of PML is likely essential for T cell activation, inhibit of PML sumoylation ( and nuclear body localization ) interfered with T cell proliferation.
author2 Ming-Zone Lai
author_facet Ming-Zone Lai
Wan-ni Yu
兪宛妮
author Wan-ni Yu
兪宛妮
spellingShingle Wan-ni Yu
兪宛妮
The Role of PML in T cell Activation and Apoptosis
author_sort Wan-ni Yu
title The Role of PML in T cell Activation and Apoptosis
title_short The Role of PML in T cell Activation and Apoptosis
title_full The Role of PML in T cell Activation and Apoptosis
title_fullStr The Role of PML in T cell Activation and Apoptosis
title_full_unstemmed The Role of PML in T cell Activation and Apoptosis
title_sort role of pml in t cell activation and apoptosis
publishDate 2002
url http://ndltd.ncl.edu.tw/handle/03996022729132045932
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