Identification of Novel Transcriptional Co-regulators of Lef1

• Main Authors: Hsu, Ju-Chun, 徐如君
• Other Authors: Lin, Young-Sun
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/18331524397278518898

碩士 === 國立臺灣大學 === 分子醫學研究所 === 90 === Wnt molecules control numerous developmental processes by altering specific gene expression patterns, and deregulation of Wnt signaling may lead to cancer. Upon Wnt stimulation, β-catenin accumulates and interacts with T cell factor/Lymphocyte enhancer binding factor (Tcfs) to activate target genes. Tcfs are required for establishing the embryonic body plan, specifying cell fate, and regulating cell proliferation and survival. Intriguingly, Tcfs participate in these cellular processes in a bimodal fashion: activating processes in one subset of cells, while simultaneously repressing the same function in a different subset. In search of additional regulatory cofactors of Lef1, we performed yeast two-hybrid screening using Lef1-△N105 as bait. Three proteins, ZF1, KIAA0941and HIPK3, were identified. The full-length of ZF1, KIAA0941 and HIPK3 were shown to interact with full-length lef1 in GST pull-down assays. KIAA0941 was shown to interact with Lef1 and repress Lef1-driven transcriptional activity in vivo.