Effects of Clomiphene Citrate on the mRNA Expression of Estrogen and Progesterone Receptors in Endometrium

• Main Authors: Ko-Chen Lin, 林克臻
• Other Authors: Yu-Shih Yang
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/07946973851427970158

碩士 === 國立臺灣大學 === 臨床醫學研究所 === 90 === Background and purpose: Clomiphene citrate is a most common agent for induction of follicle growth and ovulation. However, prolonged anti-estrogenic effects of clomiphene on endometrial receptivity and cervical mucus let the pregnancy rate much lower than expected. The present study aimed to recognize whether the detrimental effect on endometrial receptivity is due either to the direct effect of clomiphene on the steroid receptors or to the reduction of the receptor concentrations in the endometrium. Methods: A total of 25 normal cyclic patients (with five subjects among them as control group) received transvaginal ultrasound, endometrial biopsies and blood sampling in the preovulatory and mid-luteal phases after clomiphene administration 100 mg/day from cycle days 5 to 9 in the clomiphene group. Messenger RNA (mRNA) levels of estrogen receptor a (ERa), estrogen receptor b (ERb) and progesterone receptor (PR) in the biopsied endometrium were quantified using TaqMan reverse transcription-polymerase chain reaction (RT-PCR) assay. Serum levels of reproductive hormone (such as follicle-stimulating hormone (FSH), luteinizing hormone (LH), estrogen (E2) and progesterone (P4)) were derived form blood sampling. Number of follicles and endometrial condition (endometrial thickness and echogenic pattern) were collected from transvaginal ultrasound Results: The number of follicles and serum E2 concentration from the clomiphene group was significantly higher than that of the control group. There were no significant differences between the two groups in ERa, ERb, relative ratio of ERa to ERb and PR mRNA levels whether in the preovulatory and the mid-luteal phases or between the phases. Conclusions: Our results suggest that clomiphene seems to have a detrimental effect on the endometrium and there is no alteration on the mRNA expression of endometrial ERa, ERb and PR after clomiphene administration whether in the preovulatory phase, the mid-luteal phase, or between the two phases.