| Summary: | 碩士 === 國立臺灣大學 === 獸醫學研究所 === 90 === Chicken coccidia is a protozoan parasite of the genus Eimeria. It is economically important that causes loses in the poultry industry globally. The various species can be distinguished on the basis of morphology of the oocyst and the location of parasite within intestinal cells, but these criteria could be unreliable. Arbitrarily-primed PCR has been utilized to fingerprint avian Eimeria species, but this method does not always give reproducible results due to low specificity. For this reason, PCR assays have been employed for the detection of Eimeria species in this study. Five sets of primer derived from the internal transcribed spacer 1 (ITS-1) regions were used to distinguish the five Eimeria species including Eimeria tenella, E. necatrix, E. brunetti, E. acervulina and E. maxima. We use vaccine as template for the five species. The similarities of five species sequences between the vaccine and Genbank are 94%~100%. Analysis for E. tenella ITS-1 partial sequence of Taiwan and Genbank, the similarity is 99.6%. PCR sensitivity test of E. tenella in Taiwan is 50 oocysts. Five sets of primer won''''t amplify any non-specific band of chicken genome or intestinal contents.
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