| Summary: | 碩士 === 國立臺灣大學 === 園藝學研究所 === 90 === Maize is the world second largest GM-crop, and under Taiwan food regulations, the labeling of maize foods with 5 % or above GM-maize content are needed to be carried out compulsorily in 2003. In this study, we have investigated three GM-maize line by using PCR. First, four Zel primer pairs were designed for amplification of specific maize DNA for the investigation of DNA degradation during processing. The results showed that some DNA from can has degraded lower than 329 bp during processing steps, whereas frozen maize products DNA retained at length of 941 bp. For 6 months normal and frozen storage experiments, no DNA degradation been observed. Thus, most of the degradation is due to heat treatment during the processing. Few market available maize cans also been investigated, it is revealed that different processing conditions resulted different extents of degradation. Specific primers targeting for cryIA(b) of MON810 and Bt11, and bar of T25 were also designed for the detection of GM-maize. Primer pair of MON 810 YHS0301/YCR0301 is found to be with the sensitivity of 1.0 %, whereas primer pair of Bt11 YBt11adh6-0331/YBt11cry-0331 with the sensitivity of 2.0 %. Specific primers were found to have similar sensitivity with Zel primer pairs, thus, it is suitable for routine detection use. As for market products investigation, four maize cans (brand A, B, C and D) and two frozen maize (brand E, F, G and H) were found to contain MON810 and T25 GM-maize.
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