Molecular cloning of chromosome condensation related genes, Nek2 and Ran, in the carp oocytes

• Main Authors: HSIN-YI CHEN, 陳心怡
• Other Authors: Ferng-Chun Ke
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/49675607187738481089

碩士 === 國立臺灣大學 === 動物學研究所 === 90 === Chromosome condensation and spindle formation are the principle phenomena of mitosis. Compared with mitosis, meiosis has the particular characters in chromosome configuration and spindle behavior. However, chromosome condensation and spindle formation are still necessary meiotic process. Because of the different between mitosis and meiosis, the meiotic machine must be modified. Nek2 and Ran had be shown to involve in chromosome condensation. Furthermore, Ran participates in spindle formation. On the other hand, Mos knockout data implied that the vertebrate meiosis protein is related to chromosome status and microtubule dynamics. For the sake of completing the separation, cell chromosome condensation components have to coordinate, via either direct interaction or indirect communication. To investigate the characteristic of meiosis molecular mechanism, the cDNA of Nek2 and Ran genes had been cloned from the carp ovary. The carp Nek2 recombinant protein expressed in bacteria was used to produce Nek2 antiserum. Combining with the previous cloned Mos, it will be the basis of in vitro assay system. When expressing Mos recombinant protein, we observed that the recombinant protein tended to aggregation. To inquiry if this is Mos intrinsic characteristic, kinase-dead and C-terminal truncated mos mutant were also expressed in bacteria. In the expect of grasping Mos property, obtaining active expression protein and establishing a in vitro assay system to deliberate the connection between Mos, Nek2 and Ran in the future.