Development of Activity Probes for Glycosidases and Their Applications in Proteomics

碩士 === 國立臺灣大學 === 化學研究所 === 90 === The application of activity probes in proteomics, including the distribution and specificity of various glycosidases, has been developed recently. The design of activity probes is based on suicide inhibition of enzymes. The synthesis of ac...

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Main Authors:	Charng-Sheng Tsai, 蔡長昇
Other Authors:	Lee-Chiang Lo
Format:	Others
Language:	zh-TW
Published:	2002
Online Access:	http://ndltd.ncl.edu.tw/handle/40904381554177814263

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spelling	ndltd-TW-090NTU000650102015-10-13T14:38:05Z http://ndltd.ncl.edu.tw/handle/40904381554177814263 Development of Activity Probes for Glycosidases and Their Applications in Proteomics 醣水解酵素活性標示之開發及其在蛋白質體學上的應用 Charng-Sheng Tsai 蔡長昇碩士國立臺灣大學化學研究所 90 The application of activity probes in proteomics, including the distribution and specificity of various glycosidases, has been developed recently. The design of activity probes is based on suicide inhibition of enzymes. The synthesis of activity probe contains:Glycosidic bond formation, Hydrogenation, Esterification, Bromination, Hydrolysis, Fluorination, Peptide bond formation. From the signals of 19F NMR, we observed the rate of activity probes with glucosidase and formation of 1,6-elimination. In addition, we also discuss the specific coupling glucosidase by SDS-PAGE. Lee-Chiang Lo 羅禮強 2002 學位論文 ; thesis 173 zh-TW
collection	NDLTD
language	zh-TW
format	Others
sources	NDLTD
description	碩士 === 國立臺灣大學 === 化學研究所 === 90 === The application of activity probes in proteomics, including the distribution and specificity of various glycosidases, has been developed recently. The design of activity probes is based on suicide inhibition of enzymes. The synthesis of activity probe contains:Glycosidic bond formation, Hydrogenation, Esterification, Bromination, Hydrolysis, Fluorination, Peptide bond formation. From the signals of 19F NMR, we observed the rate of activity probes with glucosidase and formation of 1,6-elimination. In addition, we also discuss the specific coupling glucosidase by SDS-PAGE.
author2	Lee-Chiang Lo
author_facet	Lee-Chiang Lo Charng-Sheng Tsai 蔡長昇
author	Charng-Sheng Tsai 蔡長昇
spellingShingle	Charng-Sheng Tsai 蔡長昇 Development of Activity Probes for Glycosidases and Their Applications in Proteomics
author_sort	Charng-Sheng Tsai
title	Development of Activity Probes for Glycosidases and Their Applications in Proteomics
title_short	Development of Activity Probes for Glycosidases and Their Applications in Proteomics
title_full	Development of Activity Probes for Glycosidases and Their Applications in Proteomics
title_fullStr	Development of Activity Probes for Glycosidases and Their Applications in Proteomics
title_full_unstemmed	Development of Activity Probes for Glycosidases and Their Applications in Proteomics
title_sort	development of activity probes for glycosidases and their applications in proteomics
publishDate	2002
url	http://ndltd.ncl.edu.tw/handle/40904381554177814263
work_keys_str_mv	AT charngshengtsai developmentofactivityprobesforglycosidasesandtheirapplicationsinproteomics AT càizhǎngshēng developmentofactivityprobesforglycosidasesandtheirapplicationsinproteomics AT charngshengtsai tángshuǐjiějiàosùhuóxìngbiāoshìzhīkāifājíqízàidànbáizhìtǐxuéshàngdeyīngyòng AT càizhǎngshēng tángshuǐjiějiàosùhuóxìngbiāoshìzhīkāifājíqízàidànbáizhìtǐxuéshàngdeyīngyòng
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Development of Activity Probes for Glycosidases and Their Applications in Proteomics

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