| Summary: | 碩士 === 國立清華大學 === 生命科學系 === 90 === Japanese encephalitis virus (JEV) is found throughout Asia from India in the west to Japan in the east. JEV envelope protein plays an important role in cell receptor binding, immunological response, virion assembly and fusion activity at low pH state. The domain III of JEV envelope protein is a 111 amino acid protein with a molecular weight of 12 kDa. The backbone sequential assignments were determined from six triple resonance experiments (HNCA, HNCO, HN(CO)CA, HN(CA)CO, CBCANH, and CBCA(CO)NH). Proton side chain assignments were obtained from TOCSY-HSQC, H(CC)(CO)NH, HCCH-TOCSY and HCCH-COSY. 3JHNHA coupling constants were accomplished based on HNHA experiment. Moreover, hydrogen-deuterium exchange experiments and backbone dynamics experiments were achieved. Secondary structures were identified via NOE correlations, amide exchange, 3JHNHA coupling constants, and CSI analysis. This protein is comprised of seven beta strands and shared as an immunoglobulin-G like domain.
|
|---|
