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• Main Authors: Wong Yung-Hong, 翁永弘
• Other Authors: Liu Li-Lian
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/87091330681635113678

碩士 === 國立中山大學 === 海洋生物研究所 === 90 === Abstract C-Phycocyanin (C-PC), a water-soluble protein pigment, is one of the major constituents of Spirulina platenisi,. Which is a blue green algae and used in many countries as dietary supplements.The C-PC used in present study is a phycobiliprotein, and consist of two distinguishable protein subunits designated as α and β subunits. The aim of this study was to screen pharmacological effects including analgesic, anti-inflammatory and hepatoprotective effects of C-PC in an animal model. Concomitantly, the antioxidant effect of C-PC was measured to confirm its free radical scavenger activity. The analgesic effect of C-PC measured by tail-flick test shown the latency (sec) of control mice is approximately 4-5 sec. After administration different doses of C-PC (0.1, 1.0, 10 mg/kg), the latency resulted in a dose-dependent phenomenon and prolong from 4 sec to 4.5, 5.6, and 6.9 sec, respectively. The anti-inflammatory effect of C-PC was analyzed in the λ –carrageenan injected rat. The hind paw edema percentage change by injection of different doses (0.1~1.0 mg/kg) of C-PC, our results have indicated that C-PC possessed a dose-dependent inhibitory effect on carrageenan-induced edema formation. Intraperitoneal (i.p.) injection of different dose (0.01,0.1 1, 1.0 mg/kg) of C-PC on CCl4 (0.3 ml/kg) challenged rats have indicated that C-PC possessed a significant hepatotoprotective effect and significant decrease the serum levels of glutamate pyruvate transaminase (SGPT) and serum glutamate oxyloacetic transaminase (SGOT). Antioxidant effect of C-PC was measured by both xanthine oxidase method, and cytochrome c method. The IC50 of C-PC measured by xanthine oxidase method is 7.23 ±0.21 mg/ml. The IC50 value of C-PC estimated by cytochrome c method is approximately 6.1±0.74 mg/ml. Moreover, the direct active oxygen (·O2-) and hydrogen oxide (·OH) radical scavenging (SOD-like) activity was also confirmed by using advance electron spin resonance (ESR) method. We used the spin-trapping technique to evaluate free radical scavenging ability of C-PC. The IC50 (·O2- ) and (·OH) scavenging activities are 1.08 × 103 unit/g and 7.11 unit/g. Our results have shown the superoxide radical (·OOH) scavenging activity of C-PC is approximately 3.28 mg/ml. By contrast, IC50 of the hydroxyl radical (·OH) scavenging activity of C-PC is 4.75 mg/ml. Human hepatoma cells (HepG2 and 2.2.15 cells) and rat glioma C6 cells were cocultured with different concentration of C-PC (1.0, 10 and 20 μg/ml)for 72 hours, the cell cycle regulation of C-PC was measured by flow cytometer. We found the C-PC possessed a G0/G1 phase-arrest effect on all of the three kind cancer cell line. Whereas, the cell cycle modulations of C-PC are more significant in human hepatoma cells. In conclusion, both our pharmacological screening tests and antioxidant bioactivity assay have indicated that C-PC is a potential antitumor, anti-inflammatory and analgesic agent.