| Summary: | 碩士 === 國立屏東科技大學 === 熱帶農業研究所 === 90 === Tissue-cultured cucumber (Cucumis sativus L.) roots and spores of vesicular-arbuscular mycorrhizal fungus Glomus fasciculatum (Thaxter sensu Gerd.) Gerd. & Trappe were used to study the feasibility of asceptic symbiotic culture. The proper media for cucumber root culture, the method for spore surface sterilization, the spore germination-enhanced chemicals and the proper media strength for symbiotic culture were examined. The spores were co-cultured with the tissue-cultured roots organs. The results showed that the range of media strength for culturing cucumber roots as host was between 1/4 to 1/12 for MS solution, and 0.01 to 0.05 ppm for 2,4-D. Spores attained 60 % germination rate and had the least contamination rate when using 0.03 % sodium hypochlorite as disinfectant for 7 min and followed by 0.05 % hypochlorite for 8 min. Adding quercetin at 3-6 ppm could enhance spore germination and hyphal growth immensely. The spore germination and hyphal growth would be retarded when MS medium strength > 1/12, 2,4-D >0.01 ppm and nitrogen concentration >1/12 MS. Sugar and phosphorus had little influence on spore germination and hyphal growth.
The results observed from the symbiotic culture showed that the growth direction of hypha was unpredictable and not always grew toward the roots, so there was no tropism appeared on germinated spores. Besides, the medium strength for symbiotic culture was often too high for spore germination and, on the other hand, was too low for cucumber roots growth. Under this condition that the proper strength of culture medium was not easy to match spore and root growth, the feasibility which spores and roots form the symbiont would be affected.
|
|---|
